//Big Dreams ♥

De'Owner

Hey cutie ;) Always stay cute ?
Welcome to the blog of mine . And watch your behavior
And i meant it!*wink*

Tagboard

Sing-A-Song ~

Quotes

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" I able to tear away a part of me and let you go."

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Choosing solvent system

Isocratic elution

1 solvent or 1 mixture of solvent of constant composition during elution of compound

- Commonly used for routine analysis of 1 or 2 compound in a single run

➤ This method employs 1 mobile phase (1 solvent)

- Polar analyte might elutes (keluar) very quickly while the non polar compound will left the

phase very slow

- This will takes longer time to run

➤A mixture of 2 mobile phases with same composition

- 90% polar 10% non polar

- better run time

Gradient elution

2 or more solvents programmed to change composition of POLARITY during elutions of compound

- This method employs 2 or more solvent system that differ significantly in polarity

- The mobile phase is programmed to change in composition during elution

a bit like temperature programming in GC

- used to shorten the retention time of strongly-retained species

➤ When polar compound has left the stationary phase . The mobile phase is programmed to non polarity