//Big Dreams ♥

De'Owner

Hey cutie ;) Always stay cute ?
Welcome to the blog of mine . And watch your behavior
And i meant it!*wink*

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Sing-A-Song ~

Quotes

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" I able to tear away a part of me and let you go."

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Detection in HPLC

REFRACTIVE INDEX DETECTOR

The solvent has refractive index

the presence of analyte will change the refractive index

NON-SPECIFIC because it doesn't specify the species

UV ABSORPTION

Most compound have some absorption in the UV

- Use solvent with no absorption

Some detector is single wavelength ( 254nm)

NON-SPECIFIC because no spectral resolution

But spectra of analytes can be recorded (UV- visible spectroscopy) with very specific kind of spectrometer (diode array)

Notes to UV-visible spectroscopy